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qPCR Is a Sensitive and Rapid Method for Detection of Cytomegaloviral DNA in Formalin-fixed, Paraffin-embedded Biopsy Tissue
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A simple method using PCR for direct sequencing of genomic DNA from frozen tumor tissue embedded in optimal cutting

Y Sun1, G Hegamyer, N H Colburn

  • 1BCDP, Program Resources Inc./DynCorp, National Cancer Institute, Frederick Cancer Research and Development Center, MD 21702.

Biotechniques
|May 1, 1992
PubMed

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DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...