Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Development of an individual-specific autoantibody (ISA) protein microarray.

Stewart J Lebrun1, Edgar Suquilanda, Wasinee N Petchpud

  • 1Miragene Inc., 5475 E. La Palma Ave, Anaheim, CA 92807, USA. lebrun@miragene.com

Biotechnology and Applied Biochemistry
|May 21, 2004
PubMed
Summary

We developed a new protein microarray for individual-specific autoantibody (ISA) identification. This method improves reliability by semi-purifying proteins, overcoming limitations of previous ISA strip assays.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Comparison of endoscopic treatment for bladder neck contracture: a single centre study and review of literature.

The Canadian journal of urology·2026
Same author

Defining the role of multiparametric MRI in predicting prostate cancer extracapsular extension.

World journal of urology·2024
Same author

Retroperitoneal Robotic Partial Nephrectomy with the Hugo RAS System.

European urology·2023
Same author

Initial experience of robot-assisted partial nephrectomy with Hugo™ RAS system: implications for surgical setting.

World journal of urology·2023
Same author

Bowel Perforation after Extracorporeal Wave Lithotripsy: A Review of the Literature.

Journal of clinical medicine·2023
Same author

Modeling the antioxidant properties of the eye reduces the false-positive rate of a nonanimal eye irritation test (OptiSafe).

Toxicology in vitro : an international journal published in association with BIBRA·2021

Area of Science:

  • Biochemistry
  • Immunology
  • Proteomics

Background:

  • Autoantibodies are crucial biomarkers for disease identification.
  • Previous methods for autoantibody profiling, like ISA strips, faced challenges with reliability due to protein separation and quality control variability.
  • Developing a more robust assay is essential for accurate biological sample identification.

Purpose of the Study:

  • To develop and validate a novel protein microarray for individual-specific autoantibody (ISA) identification.
  • To address and overcome the limitations of existing ISA strip methods, particularly batch-to-batch production inconsistencies.
  • To establish a more reliable and reproducible assay for identifying biological samples based on unique autoantibody profiles.

Main Methods:

  • Semi-purification and column chromatography of human proteins for large-batch separation.

Related Experiment Videos

  • Development of an ISA-specific protein microarray utilizing these purified proteins.
  • Comparison of serum titres between the new ISA protein microarray and the original ISA strip method.

    • Main Results:

    • The ISA protein microarray demonstrated comparable serum titres to the ISA strip assay.
    • The new microarray approach effectively circumvents batch-to-batch production issues associated with SDS/PAGE.
    • Semi-purification and chromatography improved protein separation and assay consistency.

    Conclusions:

    • The developed ISA protein microarray offers a more reliable and reproducible method for autoantibody identification.
    • This advancement addresses key limitations of previous ISA strip technologies, enhancing assay robustness.
    • The new microarray platform holds promise for accurate biological sample identification and biomarker discovery.