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Comparison of IL-6-induced STAT1 activation in different responsive cells
Ji-Yan Zhang1, Yan Li, Yun-Fang Ren
1Department of Molecular Immunology, Institute of Basic Medical Sciences, Academy of Military Medical Sciences, Beijing 100850,China.
Aim:
To investigate the activation status of signal transducer and activator of transcription 1 (STAT1) induced by interlerkin-6 (IL-6) in different responsive cells.
Methods:
Immunoblot analysis was performed with antibodies against STAT1 and tyrosine-phosphorylated STAT1.
Results:
It's found that IL-6 had growth-promoting effects on 7TD1 and TF1 cells but had little effect on the tyrosine-phosphorylation of STAT1. On the other hand, IL-6 activated STAT1 in a dose and time-dependent manner in M1, R2 and U937 cells which underwent growth arrest and macrophage differentiation upon IL-6 induction.
Conclusion:
These results suggest that IL-6-induced STAT1 activation might be associated with growth-inhibitory effects, and our data might help to explain why IL-6 exerts distinct biological effects in different target cells.