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[Interaction between p53 and HSP70 in human hepatocellular carcinoma tissues]
Chong-Wei Cui1, Shou-Jing Yang, Yan-Ping Liu
1Department of Pathology, Fourth Military Medical University, Xi'an 710032, China.
Aim:
To investigate the interaction between p53 and heat shock protein70 (HSP70) in human hepatocellular carcinoma tissues.
Methods:
HSP70 and p53-double positive samples were screened by imunohistochemical staining from twelve human hepatocellular carcinoma tissues, and the tissue homogenate was prepared. Coimmunoprecipitation was performed by using either anti-HSP70 mAb or anti-p53 mAb. The immunoprecipitate was analysed by SDS-PAGE and Western blot.
Results:
Immunohistochemical staining detection showed that three samples out of twelve cases were double positive for both HSP70 and p53. p53 protein existed in the immunoprecipitate of anti-HSP70 mAb, while there was HSP70 protein in the immunoprecipitate of anti-p53 mAb.
Conclusion:
HSP70 and p53 exist as a complex in human hepatocellular carcinoma tissues, which will provide a new route for studying the pathogenesis and immunotherapy of human hepatocellular carcinoma.
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