Cell-cell contact and anatomical compatibility in stromal cell-mediated HSC support during development

Hematopoietic stem cells (HSCs) are able to generate the wide variety of blood cells found in the adult and are maintained in the bone marrow (BM) stromal microenvironment. In the aorta-gonads-mesonephros (AGM), which autonomously generates the first HSCs, the stromal microenvironment is largely uncharacterized. We have previously made an extensive panel of stromal clones from AGM subregions and have found that clones from the urogenital ridges (UG) provide the most potent support for adult BM HSCs. However, it is unknown to what extent the stroma from this developmentally and anatomically distinct microenvironment can support HSCs from other regions of the embryo, such as yolk sac. Moreover, it is unknown whether cell-cell contact is necessary in this microenvironment. Here, we show that the HSCs from the embryonic aorta are the most potently supported HSCs in UG stromal clone co-cultures and that contact is required for the maintenance and expansion of embryo-derived HSCs.