Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Controlled, scalable embryonic stem cell differentiation culture.

Stephen M Dang1, Sharon Gerecht-Nir, Jinny Chen

  • 1Institute of Biomaterials and Biomedical Engineering, Department of Chemical Engineering and Applied Chemistry, University of Toronto, Toronto, Ontario, Canada.

Stem Cells (Dayton, Ohio)
|May 22, 2004
PubMed
Summary

Embryonic stem cell (ESC) agglomeration inhibits growth, but encapsulating ESCs in agarose capsules enables scalable hematopoietic progenitor cell production in bioreactors.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Gene syntax defines supercoiling-mediated transcriptional feedback.

Science (New York, N.Y.)·2026
Same author

Tunable differentiation of human CD4<sup>+</sup> and CD8<sup>+</sup> T cells from pluripotent stem cells.

Cell stem cell·2026
Same author

Innate lymphoid cells in the spotlight: from biomarkers to blueprint for innovative immunotherapy.

Frontiers in immunology·2025
Same author

Microbial dysbiosis sculpts a systemic ILC3/IL-17 axis governing lung inflammatory responses and central hematopoiesis.

Mucosal immunology·2025
Same author

Gene syntax defines supercoiling-mediated transcriptional feedback.

bioRxiv : the preprint server for biology·2025
Same author

Tracking the gene expression programs and clonal relationships that underlie mast, myeloid, and T lineage specification from stem cells.

Cell systems·2024

Area of Science:

  • Stem cell biology
  • Biotechnology
  • Developmental biology

Background:

  • Embryonic stem (ES) cells are crucial for regenerative medicine.
  • Embryoid body (EB) formation and differentiation are key processes.
  • EB agglomeration in cultures inhibits cell growth and differentiation.

Purpose of the Study:

  • To investigate the mechanisms of EB agglomeration.
  • To develop a scalable method for ES cell culture and differentiation.
  • To control cell-cell interactions in bioreactors.

Main Methods:

  • Studied E-cadherin-mediated cell attachment and migration during EB agglomeration.
  • Developed a technology for mass encapsulation of ES cells in size-specified agarose capsules.
  • Utilized stirred-suspension bioreactors for scalable culture.

Related Experiment Videos

Main Results:

  • E-cadherin-mediated attachment and cell migration drive EB agglomeration.
  • Agarose capsule encapsulation controls cell-cell interactions.
  • Scalable production of hematopoietic progenitor cells was achieved in bioreactors.

Conclusions:

  • Controlling EB agglomeration is vital for efficient ES cell culture.
  • Agarose encapsulation offers a scalable solution for ES cell differentiation.
  • This technology facilitates the generation of therapeutic cells in a controlled environment.