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Related Experiment Videos

FISH mapping for helminth genome.

Hirohisa Hirai1, Yuriko Hirai

  • 1Center for Human Evolution Modeling Research, Department of Cellular and Molecular Biology, Primate Research Institute, Kyoto University, Inuyama, Aichi, Japan.

Methods in Molecular Biology (Clifton, N.J.)
|May 22, 2004
PubMed
Summary

This chapter details fluorescence in situ hybridization (FISH) techniques for genome mapping in schistosomes and filariae. It covers methods for bacterial artificial chromosome (BAC) and yeast artificial chromosome (YAC) clones, aiding in gene localization.

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Area of Science:

  • Genomics
  • Molecular Biology
  • Parasitology

Background:

  • Genome projects for schistosomes and filariae require advanced mapping techniques.
  • Fluorescence in situ hybridization (FISH) is a powerful tool for chromosome analysis.

Purpose of the Study:

  • To introduce basic and advanced FISH techniques for genome mapping.
  • To provide practical guidance on using FISH with bacterial artificial chromosome (BAC) and yeast artificial chromosome (YAC) clones.

Main Methods:

  • Chromosome preparation and DNA labeling for FISH.
  • Hybridization, microscopy, and localization of BAC clones.
  • Detailed protocols for FISH in parasitic worms.

Main Results:

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  • Established FISH protocols applicable to schistosome and filaria genomes.
  • Demonstrated successful localization of BAC clones on chromosomes.
  • Provided a comprehensive guide for researchers.

    • Conclusions:

    • FISH is an effective method for high-resolution genome mapping in parasitic organisms.
    • The described techniques facilitate gene identification and chromosome analysis in schistosomes and filariae.
    • This work supports ongoing and future genomic research in these parasite groups.