Related Experiment Video
Updated: Aug 24, 2026
Use of Freeze-thawed Embryos for High-efficiency Production of Genetically Modified Mice
Published on: April 2, 2020
Update of mouse microsatellite database of Japan (MMDBJ)
Takahiro Sakai1, Ikuo Miura, Satomi Yamada-Ishibashi
1Department of Laboratory Animal Science, The Tokyo Metropolitan Institute of Medical Science (Rinshoken), Japan.
Abstract:
We updated a database of microsatellite marker polymorphisms found in inbred strains of the mouse, most of which were derived from the wild stocks of four Mus musculus subspecies, M. m. domesticus, M. m. musculus, M. m.castaneus and M. m. molossinus. The major aim of constructing this database was to establish the genetic status of these inbred strains as resources for linkage analysis and positional cloning. The inbred strains incorporated in our database are A/J, C57BL/6J, CBA/J, DBA/2J, SM/J, SWR/J, 129Sv/J, MSM/Ms, JF1/Ms, CAST/Ei, NC/Nga, BLG2/Ms, NJL/Ms, PGN2/Ms, SK/CamEi and SWN/Ms, which have not or have only been poorly incorporated in the Whitehead Institute/MIT (WI/MIT) microsatellite database. The number of polymorphic microsatellite loci incorporated in our database is over 1,000 in all strains, and the URL site for our database is located at http:// www.shigen.nig.ac.jp /mouse/mmdbj/mouse.html.
Insights
This study presents an updated mouse microsatellite database, crucial for understanding genetic diversity in wild mouse strains. The database aids researchers in genetic mapping and positional cloning efforts.
Area of Science:
- Genetics
- Genomics
- Animal Models
Background:
- Microsatellite markers are essential for genetic analysis in inbred mouse strains.
- Understanding the genetic status of mouse strains is vital for research reproducibility.
- Existing databases have limited coverage of certain wild-derived inbred mouse strains.
Purpose of the Study:
- To create a comprehensive database of microsatellite polymorphisms in diverse inbred mouse strains.
- To facilitate genetic linkage analysis and positional cloning using well-characterized mouse resources.
- To improve the genetic characterization of mouse strains derived from wild Mus musculus subspecies.
Main Methods:
- Compilation and update of microsatellite marker polymorphism data.
- Inclusion of data for 16 specific inbred mouse strains, many previously underrepresented.
- Focus on strains derived from Mus musculus domesticus, M. m. musculus, M. m. castaneus, and M. m. molossinus.
Main Results:
- An updated database containing over 1,000 polymorphic microsatellite loci across all included strains.
- Integration of data for strains like A/J, C57BL/6J, DBA/2J, and MSM/Ms.
- Improved accessibility to genetic information for underutilized mouse resources.
Conclusions:
- The developed database serves as a valuable genetic resource for the research community.
- Enhanced genetic characterization of mouse strains supports more accurate downstream analyses.
- The database promotes the effective use of diverse inbred mouse strains in genetic studies.
