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Purification of goat immunoglobulin G by immobilized metal-ion affinity using cross-linked alginate beads

Sulakshana Jain1, Munishwar N Gupta

  • 1Chemistry Department, Indian Institute of Technology, Delhi, Hauz Khas, New Delhi 110016, India.

Biotechnology and Applied Biochemistry
|May 25, 2004
PubMed

The alginate beads obtained by cross-linking of the polymer by epichlorohydrin were charged with Cu(II). The copper-charged beads could be directly used as a immobilized-metal-affinity-chromatographic medium for purification of goat IgG. The best results in the packed-bed mode were obtained by using beads charged with 95.4 micromol/ml Cu(II). We found that we could recover 97.4% IgG with an 8-fold purification.

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