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Updated: Aug 24, 2026
Peptide Scanning-assisted Identification of a Monoclonal Antibody-recognized Linear B-cell Epitope
Published on: March 24, 2017
[Preparation and identification of polyclonal antibody to B cell epitope domain of TRP-1]
Ting-Hui Li1, Tian-Wen Gao, Chun-Ying Li
1Department of Dermatology and Venerology, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China.
Aim:
To prepare the polyclonal antibody to B cell epitope domain of TRP-1 and apply it to study the immunotherapy of vitiligo and melanoma.
Methods:
The fusion protein PRSETA/TRP-1 was expressed in E.coli, the polyclonal antibody was prepared by immunizing a rabbit with the protein. The qulity of the antibody was identified by ELISA and Western-blot.
Results:
(1)The pRSEA/TRP-1 fusion protein was correctly expressed;(2)the polyclonal antibodies to the B cell eoitope domain of TRP-1 were obtained; (3)the rabbit antiserum containing of polyclonal antibody showed high titer and possessed good binding activity to 6His-TRP1 expressed by Pichia pastoris.
Conclusion:
The polyclonal antibody could be applied to the research on B cell epitope domain of TRP-1.
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