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Updated: Aug 24, 2026
Microfluidics in Assessing Platelet Function
Published on: November 8, 2024
[Studies on the antithrombotic effects of anti-platelet mAb SZ-21 F(ab')(2) fragment]
Bo-Jing Shao1, Yi-Min Zhao, Fei Shen
1Jiangsu Institute of Hematology,The First Affiliated Hospital to Suzhou University, Suzhou 215006, China.
Aim:
To research the biological effect of the F(ab')(2) of anti-platelet GPIIIa mAb SZ-21.
Methods:
The F(ab')(2) fragment was obtained with papain digestion for 20 hours at 4 degrees Celsius, under acid condition(pH 5.5). The products were purified through DEAE-52 ion exchange column and the first eluted peak components were verified as F(ab')(2) by SDS-PAGE. The immunoactivity and its antithrombotic effects of fragment were detected by ELISA and platelet aggregation inhibition test, respectively.
Results:
The recovery rate of the fragment was 60.32%, its immunoactivity was 0.8 x10(-9) mol/L by ELISA. The 50% effective dose (ED(50)) in the platelet aggregate inhibition test was 3.395 mg/L.
Conclusion:
The F(ab')(2) fragment was superior to intact IgG in binding to platelets and inhibiting platelet aggregation. Thus the F(ab')(2) fragment of mAb SZ-21 opens up broad prospects for its application to the clinical antithrombotic therapy.
