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Expression pattern of VEGFR-2 (Quek1) during quail development
S Nimmagadda1, P G Loganathan, J Wilting
1Institute of Anatomy and Cell Biology II, University of Freiburg, Albertstrasse 17, 79104 Freiburg, Germany.
Anatomy and Embryology
|May 25, 2004
Summary
Vascular endothelial growth factor receptor 2 (VEGFR-2) is crucial for blood and lymphatic system development. This study maps VEGFR-2 expression in quail embryos, revealing its role in mesodermal precursor cells for blood and vessel formation.
Area of Science:
- Developmental Biology
- Vascular Biology
- Molecular Biology
Background:
- Blood and lymphatic vascular system growth is regulated by vascular endothelial growth factor (VEGF) family members.
- These factors act via tyrosine kinase receptors (VEGFRs) on angioblastic cells.
Purpose of the Study:
- To analyze the expression pattern of Quail VEGFR-2 (Quek1), a key receptor in vascular development, during quail embryogenesis.
- To understand the role of VEGFR-2 expressing cells in mesodermal precursor lineages.
Main Methods:
- Whole mount in situ hybridization was employed to detect Quek1 expression patterns.
- Analysis was performed throughout various stages of quail embryonic development.
Main Results:
- Quek1 expression was observed in early embryogenesis at the caudal blastoderm, primitive streak, and head paraxial mesoderm.
- Expression was detected in somites, notochord, limb buds, and during organogenesis in pharyngeal arches and developing organs (esophagus, trachea, stomach, lungs, liver, heart, gut).
- Feather bud expression was noted from day 7 onwards.
Conclusions:
- The findings confirm the angiogenic potential of mesoderm.
- VEGFR-2 expressing cells likely represent diverse mesodermal precursor pools for hematopoietic and angiopoietic lineages.