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Interferences in hormone immunoassays.

George G Klee¹

¹Department of Laboratory Medicine and Pathology, Mayo Clinic College of Medicine, 200 First Street Southwest, Rochester, MN 55905, USA. klee.george@mayo.edu

Clinics in Laboratory Medicine

|May 26, 2004

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Immunoassays are robust, but interference can occur. Laboratories rely on clinician communication and specific tests to investigate potential immunoassay interference and ensure accurate results.

Area of Science:

Clinical Chemistry
Laboratory Medicine
Analytical Chemistry

Background:

Immunoassays are widely used for diagnostic testing.
Potential interferences can compromise immunoassay accuracy.
Identifying interference before testing is not feasible.

Purpose of the Study:

To discuss common causes of immunoassay interference.
To outline strategies for investigating suspected interference.
To highlight the importance of laboratory-clinician communication.

Main Methods:

Review of common immunoassay interference types (cross-reactivity, heterophile antibodies).
Description of investigative techniques: dilutional linearity, spiked recovery, heterophile antibody blocking, alternate technology testing.

Related Experiment Videos

Emphasis on post-analytical investigation triggered by clinical suspicion.

Main Results:

Competitive immunoassays are prone to cross-reactivity.
Immunometric assays frequently encounter heterophile antibody interference.
Investigative methods can help identify and confirm assay interference.

Conclusions:

Immunoassays are generally reliable measurement systems.
Laboratories must depend on clinicians to flag potentially erroneous results due to interference.
Systematic investigation protocols are essential for managing immunoassay interference.