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Updated: Aug 24, 2026

Reverse Yeast Two-hybrid System to Identify Mammalian Nuclear Receptor Residues that Interact with Ligands and/or Antagonists
10:51

Reverse Yeast Two-hybrid System to Identify Mammalian Nuclear Receptor Residues that Interact with Ligands and/or Antagonists

Published on: November 15, 2013

Steroid receptor action

Jan J Brosens1, Jennifer Tullet, Rana Varshochi

  • 1Faculty of Medicine, Hammersmith Hospital, Institute of Reproductive and Developmental Biology, Wolfson and Weston Research Centre for Family Health, Imperial College London, London W12 0NN, UK. j.brosens@imperial.ac.uk

Best Practice & Research. Clinical Obstetrics & Gynaecology
|May 26, 2004
PubMed

Insights

Ovarian hormones oestradiol and progesterone, via oestrogen receptor (ER) and progesterone receptor (PR), regulate gene expression. Their actions involve complex interactions with co-activators, co-repressors, and signalling pathways, influencing ovarian and uterine functions.

Area of Science:

  • Endocrinology
  • Molecular Biology
  • Genetics

Background:

  • Ovarian hormones, oestradiol and progesterone, are critical regulators of reproductive functions.
  • These hormones primarily act through specific nuclear receptors: oestrogen receptor (ER) and progesterone receptor (PR).

Purpose of the Study:

  • To summarize the molecular mechanisms underlying hormone action in target cells.
  • To highlight the diversity of factors modulating cellular responses to hormonal signals.
  • To emphasize the roles of ER and PR in ovarian and uterine physiology.

Main Methods:

  • Review of literature on nuclear receptor function and gene regulation.
  • Analysis of the interaction between steroid hormone receptors and other transcription factors.
  • Examination of cytoplasmic signalling pathways influencing receptor activity.

Main Results:

  • Oestrogen receptor (ER) and progesterone receptor (PR) are ligand-dependent transcription factors.
  • Receptor activation involves binding to gene promoter regions and recruitment of co-regulators.
  • Gene transcription modulation is influenced by interactions with other transcription factors and signalling pathways.

Conclusions:

  • Cellular response to ovarian hormones is determined by a complex interplay of factors.
  • Oestrogen receptor (ER) and progesterone receptor (PR) play pivotal roles in regulating ovarian and uterine functions through intricate molecular mechanisms.

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