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Screening for topoisomerase I binding compounds by high-performance liquid chromatography-mass spectrometry

Hong Zhang1, Qing Gu, Xinle Liang

  • 1Department of Food Science, Hangzhou University of Commerce, Hangzhou, People's Republic of China.

Analytical Biochemistry
|May 26, 2004
PubMed

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The role of the detectors in High-Performance Liquid Chromatography (HPLC) is to analyze the solutes as they exit from the chromatographic column. The detector recognizes the solute's property and generates corresponding electrical signals, which are converted into a readable graph of the detector's response versus elution time called a chromatogram at the computer. There are several types of HPLC detectors, each with its own advantages and limitations, depending on the analyte properties and...