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Related Experiment Videos

Echinococcus multilocularis laminated-layer components and the E14t 14-3-3 recombinant protein decrease NO production

M Amparo Andrade1, Mar Siles-Lucas, Elsa Espinoza

  • 1Laboratorio de Parasitología, Facultad de Farmacia, Universidad de Salamanca, Avda, Campo Charro s/n 37007 Salamanca, España, Spain.

Nitric Oxide : Biology and Chemistry
|May 26, 2004
PubMed
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Echinococcus parasites modulate host nitric oxide (NO) production. Specific parasite components, particularly E. multilocularis 14-3-3 protein, were found to suppress NO production in stimulated macrophages.

Area of Science:

  • Parasitology
  • Immunology
  • Molecular Biology

Background:

  • Echinococcus species cause echinococcosis, impacting host immune responses.
  • Nitric oxide (NO) production by macrophages is crucial in host defense.
  • The specific parasite components that modulate NO production remain largely uncharacterized.

Purpose of the Study:

  • To investigate the effects of Echinococcus granulosus and Echinococcus multilocularis metacestode components on nitric oxide production by rat alveolar macrophages.
  • To identify specific parasite molecules responsible for modulating macrophage NO production.

Main Methods:

  • In vitro culture of rat alveolar macrophages.
  • Exposure to defined structural and metabolic components from E. granulosus and E. multilocularis metacestodes.

Related Experiment Videos

  • Measurement of nitric oxide production, with and without prior lipopolysaccharide (LPS) stimulation.
  • Analysis of inducible nitric oxide synthase (iNOS) mRNA expression.

    • Main Results:

    • None of the tested Echinococcus antigens stimulated macrophage NO production independently.
    • Soluble components from E. multilocularis laminated layer (LL) and cyst wall (CW), as well as E. multilocularis 14-3-3 protein (E14t), inhibited LPS-induced NO production.
    • This inhibition was dose-dependent and also affected iNOS mRNA levels.

    Conclusions:

    • Echinococcus metacestode components, particularly from E. multilocularis, can suppress macrophage NO production.
    • The E. multilocularis 14-3-3 protein is identified as a key component contributing to this suppressive effect.
    • These findings shed light on parasite immune evasion strategies in echinococcosis.