[Purification and characterization of lipoteichoic acid from Streptococcus Sobronus]

Lipoteichoic acid (LTA) is amphipathic molecule found in cell surface of gram-positive bacteria.Although there were some hypothese about adhesion of LTA,the role of LTA in bacterial pathogenicety is still unclear.For this reason,a seria studies of LTA were carried out.In this study LTA was extracted by hot aqueous phenol from Strptococcus Sobronus 6715 grown in tryptic soy both supplemented with 5% sucrose for 17 hours,and was purfied on gel filtration by Sepharose 6B,chloroform-methanol precipitation and chromtography on DEAE-Sephacel.The purified LTA was characterized by FT-IR and 1H-NMR with standard sample of LTA (Sigma Co) as control.The results showed that LTA contains alanine ester and glycosyl residues which take important role in the amphipathic molecule.