[Chemical analysis of lipopolysac-charides derived from oral anacerobes]

Lipopolysaccharides were isolated from six oral anaerobes(Va.Bg.Bf.Fn.Aa.Co.)by the hot phenol-water procedure and purified by PCP method.The six species can be all extracted their LPS with different yields (from 0.70 to 2.83% of cell dried weight).Comparable of LPS with the enterobacteriaceae(Sm.),they were the constructed with hexosamine,fatty acids and phosphorus etc.On the other hand,they showed much distinct differences from the enterobacterial LPS structure as follow:(1) 2-keto-3-deoxy-D-mannooctonate(KDO) was not detected by routine procedure.(2)The amount of heptose is reltively lower.(3) Methylated and branched fatty acids were detected.These chemical characteristics may relate to biological activities and pathogenic potential of LPS and bacteria themselves.