[The study of influencing cellular dynamics of gynostemma pentaphyllum on golden hamster cheek pouch premalignancy]
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Published on: January 9, 2018
1Department of Oral Medicine, Ninth People's Hospital, School of Stomatology, Shanghai Second Medical University. Shanghai 200011,China.
Abstract:
Studying premalignancy is an important field of conquering cancer.The aim to study the mechanics of oral mucosal premalignant formation and seek effective drug which could prevent and block cancer,Gynostemma Pentaphyllum(GP) was applies to block carcinomatous conversion of golden hamster cheek pouch by Salley's way.Using light microscopic observation and proliferating cell nuclear antigen(PCNA) as well as bromodeoxyuridine(BrdU)(PCNA AND BrdU are immunohistochemistry techniques),GPs effect was observed dynamically.We found that GP could delay the presence of leukoplakia and decrease the rate of presence as well as cancer-causing rate. The positive cells of taking drug group PCNA and BrdU were lower than model group significantly and in a lower condition all the time.It is illustrated that GP has the effect of preventing cancer and inhibiting cellular dysplasia.
Insights
Gynostemma Pentaphyllum (GP) may prevent oral cancer by delaying premalignant lesions. Studies show GP reduces cell proliferation markers, suggesting it inhibits dysplasia and blocks cancer development.
Area of Science:
- Oncology
- Cancer Research
- Oral Medicine
Background:
- Studying premalignancy is crucial for cancer prevention.
- Oral mucosal premalignant lesions are a significant concern.
- Identifying effective preventive agents is a key research goal.
Purpose of the Study:
- To investigate the mechanics of oral mucosal premalignant formation.
- To evaluate Gynostemma Pentaphyllum (GP) as a potential cancer preventive agent.
- To assess GP's efficacy in blocking carcinomatous conversion.
Main Methods:
- Utilized the golden hamster cheek pouch model (Salley's method).
- Employed light microscopic observation.
- Assessed cell proliferation using immunohistochemistry techniques for proliferating cell nuclear antigen (PCNA) and bromodeoxyuridine (BrdU).
Main Results:
- Gynostemma Pentaphyllum (GP) delayed the onset of leukoplakia.
- GP significantly decreased the incidence rate and cancer-causing rate of lesions.
- The drug-treated group showed significantly lower positive cell counts for PCNA and BrdU compared to the control group.
Conclusions:
- Gynostemma Pentaphyllum (GP) demonstrates potential in preventing oral cancer.
- GP effectively inhibits cellular dysplasia and blocks carcinomatous progression.
- GP may serve as a valuable agent for cancer chemoprevention.