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Klotho is a serum factor related to human aging
Neng-ming Xiao1, Yan-ming Zhang, Quan Zheng
1Department of Biochemistry and Molecular Biology, College of Life Science, Peking University, Beijing 100871, China.
Chinese Medical Journal
|May 27, 2004
Summary
Klotho (KL) protein is present in human serum. Serum KL levels decrease with age, suggesting KL is a biomarker for human aging.
Area of Science:
- Biochemistry
- Gerontology
- Immunology
Background:
- The presence of klotho (KL) protein in human serum and its association with aging requires investigation.
- Understanding serum KL levels is crucial for aging research.
Purpose of the Study:
- To identify KL protein in human serum.
- To determine the correlation between serum KL protein levels and human aging.
Main Methods:
- A polyclonal antibody was developed to detect human secreted KL protein.
- Western blot and ELISA were employed to identify and quantify serum KL protein.
Main Results:
- A 60-kD KL protein was specifically identified in human and mouse serum via Western blot.
- ELISA analysis of individuals aged 0-91 years showed a decline in serum KL levels with increasing age.
- The age-related decrease in serum KL was consistent across sexes.
Conclusions:
- Klotho protein is detectable in human serum.
- Serum KL levels are significantly correlated with the human aging process.