Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Genes without frontiers?

D Bensasson1, J L Boore, K M Nielsen

  • 1Evolutionary Genomics Department, DOE Joint Genome Institute, 2800 Mitchell Drive, Walnut Creek, CA 94598, USA. d.bensasson@imperial.ac.uk

Heredity
|May 27, 2004
PubMed
Summary

Bacterial DNA sequence similarity acts as a barrier to foreign DNA uptake. This review explores if artificial vector homology, like pUC18, can facilitate foreign DNA insertion into bacteria.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Population genetics of the metabolically related Adh, Gpdh and Tpi polymorphisms in Drosophila melanogaster : II. Temporal and Spatial Variation in an Orchard Population.

Genetique, selection, evolution·2012
Same author

Phase II study of biweekly cetuximab in combination with irinotecan as second-line treatment in patients with platinum-resistant gastro-oesophageal cancer.

European journal of cancer (Oxford, England : 1990)·2012
Same author

Retrospective evidence for a biological cost of vancomycin resistance determinants in the absence of glycopeptide selective pressures.

The Journal of antimicrobial chemotherapy·2011
Same author

Domestication of olive fly through a multi-regional host shift to cultivated olives: comparative dating using complete mitochondrial genomes.

Molecular phylogenetics and evolution·2010
Same author

Tn1546 is part of a larger plasmid-encoded genetic unit horizontally disseminated among clonal Enterococcus faecium lineages.

The Journal of antimicrobial chemotherapy·2010
Same author

Complete sequence of Enterococcus faecium pVEF3 and the detection of an omega-epsilon-zeta toxin-antitoxin module and an ABC transporter.

Plasmid·2008

Area of Science:

  • Microbiology
  • Molecular Biology
  • Genetics

Background:

  • Bacterial DNA sequence dissimilarity is a primary barrier to non-self-transmissible genetic exchange.
  • Homologous recombination is common in bacteria and can facilitate uptake of linked foreign DNA.
  • Artificial vectors are composed of mobile DNA elements, retaining some homology to natural bacterial DNA.

Purpose of the Study:

  • To investigate if homology between artificial vectors and bacterial DNA can enable foreign DNA uptake.
  • To examine pUC18 as a model artificial vector and its potential for homology-facilitated DNA uptake.
  • To discuss the environmental persistence and potential natural homologies of recombinant DNA.

Main Methods:

  • Review of existing literature on homology-facilitated foreign DNA uptake.
  • Analysis of pUC18's homology to antibiotic resistance transposons and plasmid origins of replication.
  • Discussion of recombinant DNA disposal and environmental persistence.

Main Results:

  • Homology between artificial vectors and bacterial DNA is a potential mechanism for foreign DNA uptake.
  • pUC18 exhibits homology to elements that could facilitate insert DNA uptake.
  • The extent of homology and its impact on insert DNA utility for bacteria are key considerations.

Conclusions:

  • Vector-bacterial DNA homology can potentially enable the uptake of foreign DNA inserts.
  • Understanding these homologies is crucial for assessing the implications of recombinant DNA in bacterial populations.
  • Further research is needed to fully elucidate the role of homology in artificial DNA uptake and environmental fate.

Related Experiment Videos