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Eight gram-negative bacteria are 10,000 times more sensitive to cationic detergents than to anionic detergents
Soumitra Rajagopal1, Nicole Eis, Kenneth W Nickerson
1School of Biological Sciences, University of Nebraska, Lincoln, NE 68588-0666, USA.
Abstract:
In liquid culture, eight typical gram-negative bacteria were ca. 10,000-fold more sensitive to cationic detergents than to the anionic detergent sodium dodecyl sulfate. Cetyltrimethylammonium bromide (CTAB) was inhibitory at concentrations ranging from 0.0006% to 0.01%. Four pseudomonads able to form biofilms were ca. 1000-fold more resistant to CTAB on Luria-Bertani agar plates than they were in liquid culture. A lasI mutant of Pseudomonas aeruginosa was only able to tolerate 0.1% CTAB on Luria-Bertani agar plates but could tolerate 5% CTAB when supplemented with homoserine lactone containing culture supernatants.
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