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Induction of somatic hypermutation by antigen-specific B cell receptors in the human BL2 cell line

Florence Glaudet1, Vincent Denis, Michel Cogné

  • 1CNRS UMR 6101, Laboratoire d'Immunologie, Faculté de Médecine, Limoges, France.

European Journal of Immunology
|May 27, 2004
PubMed

The role of the B cell antigen receptor in the induction of somatic hypermutation is presently unclear. We established stable transfectants of the human BL2 cell line expressing hen-egg lysozyme-specific IgM or IgA and compared their ability to induce somatic hypermutation of the endogenous rearranged heavy-chain gene. We found that IgM and IgA were both able to induce somatic hypermutation in an antigen dose-independent manner. The mutations displayed most of the characteristics of somatic hypermutation in vivo. Notably, some replacements introduced stop codons in the coding region. Our data suggest that class-switched memory B cells may undergo somatic hypermutation. They also suggest that the transmembrane/cytoplasmic domains of the class-switched isotypes modulate the signaling and down-modulation activities of the BCR in an antigen dose-dependent manner.

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