[Effects of altered peptide ligands (APLs) of proteolipid(136-150) on T-cell clone 4B.14a]
1Department of Neurology, China-Japan Friendship Hospital, Beijing 100029, China.
Aim:
By investigating the effects of APLs of PLP(136-150) on T cell clone 4B.14a in-vitro and in-vivo, to determine the feasibility of APLs to prevent relapsing multiple sclerosis (MS) in human.
Methods:
To mimic the clinic course of relapsing MS, SJL/J female mice were first irradiated at 450R and intravenously infused with 1x10(7) resting 4B.14a T cells per mouse. Then mice were immunized with 50 microg/mouse APL for inducing passive experiment allergic encephalomyelitis (EAE). The proliferation and cytokine production of 4B.14a T cells in response to APLs were also examined.
Results:
Except 139A, 143A, 144A, 145A and 148A, other APLs triggered T cells to induce passive EAE; 4B.14a T cells well responded to the most APLs, weakly responded to 137A, 144A and 148A, but the response was suppressed by 139A.
Conclusion:
The effects of some APLs on 4B.14a T cells are different in-vitro and in-vivo. It may be feasible to select some APLs to prevent relapsing MS in human.
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