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Related Experiment Videos

SV40 in human thyroid nodules.

A Ozdarendeli1, C Camci, E Aygen

  • 1Department of Microbiology and Clinical Microbiology, College of Medicine, Firat University, Elazig, Turkey. aozdarendeli@firat.edu.tr

Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology
|May 28, 2004
PubMed
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Simian virus 40 (SV40) DNA was detected in four thyroid nodules, including two papillary thyroid carcinomas and two benign nodules. SV40 was not found in normal thyroid tissue from the same patients, suggesting a potential role in thyroid nodule development.

Area of Science:

  • Virology
  • Oncology
  • Molecular Biology

Background:

  • Simian virus 40 (SV40) is a well-established model for cell transformation and tumorigenesis research.
  • Human studies on SV40 have prompted interest in related polyomaviruses, BK virus (BKV) and JC virus (JCV), and their association with human diseases.
  • SV40 has been identified in various human samples, both cancerous and normal, with ongoing research into its oncogenic potential.

Purpose of the Study:

  • To investigate the presence of Simian virus 40 (SV40) sequences in human thyroid nodules.
  • To determine if SV40 is associated with thyroid carcinomas or benign thyroid nodules.
  • To compare SV40 detection rates in diseased thyroid tissue versus adjacent normal thyroid tissue.

Main Methods:

  • Polymerase chain reaction (PCR) was employed to detect SV40 sequences.

Related Experiment Videos

  • Samples included 99 patients with thyroid nodules, with both nodule and normal tissue collected from each.
  • DNA sequence analysis was performed on positive PCR products to confirm SV40 T antigen (Tag) region presence.

    • Main Results:

    • SV40 sequences were detected in 4 out of 99 thyroid nodules using PCR.
    • Two of the positive samples were identified as papillary thyroid carcinomas, and the other two were benign thyroid nodules.
    • No SV40 sequences were detected in the 99 corresponding normal thyroid tissue samples.

    Conclusions:

    • The findings suggest a potential association between SV40 and the development of thyroid nodules.
    • The study discusses the implications of SV40 detection in thyroid tissue for understanding its role in human disease.
    • The results contribute to the ongoing discussion regarding the horizontal transmission and oncogenic potential of SV40 in the human population.