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Updated: Aug 24, 2026

Highly Sensitive and Quantitative Detection of Proteins and Their Isoforms by Capillary Isoelectric Focusing Method
Published on: September 19, 2018
Analysis of proteins by CE, CIEF, and microfluidic devices with whole-column-imaging detection
Jiaqi Wu1, Xing-Zheng Wu, Tiemin Huang
1Convergent Bioscience Ltd., Toronto, Ontario, Canada.
Abstract:
The recently developed whole-column-imaging detection technique for capillary electrophoresis (CE) and capillary isoelectric focusing (CIEF), a commercial whole-column-imaged CIEF instrument and its standard operation protocol are introduced. Furthermore, new developments and applications of whole-column-imaging detection in protein-protein interaction study, in protein separation using microfluidic devices and CIEF methods without carrier ampholytes, as well as in 2D separation techniques are reviewed. Miniaturization of whole-column-imaging CIEF and axially illuminated fluorescence whole-column-imaging CIEF are also discussed.
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