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A CD14 promoter polymorphism is associated with CD14 expression and Chlamydia-stimulated TNF alpha production

H L Eng1, C H Wang, C H Chen

  • 1Department of Pathology, Chang Gung University and Memorial Hospital, Kaohsiung Medical Center, Kaohsiung, Taiwan, Republic of China.

Genes and Immunity
|May 28, 2004
PubMed

Insights

A CD14 gene polymorphism influences CD14 expression and TNF-alpha production, potentially impacting inflammatory responses to Chlamydia infections. This finding offers insights into innate immunity and disease risk.

Area of Science:

  • Immunology
  • Genetics

Background:

  • CD14 is a key pattern recognition receptor in innate immunity, recognizing bacterial lipopolysaccharide and triggering inflammatory responses.
  • CD14 promoter gene polymorphism (-260C>T) is linked to inflammatory disease risk.

Purpose of the Study:

  • To investigate the association between CD14 promoter gene polymorphism and CD14 expression levels.
  • To examine the impact of this polymorphism on TNF-alpha production following Chlamydia stimulation.

Main Methods:

  • Genotyping of the CD14/-260C>T promoter polymorphism in a Chinese population in Taiwan.
  • Quantification of membrane and soluble CD14 levels.
  • Measurement of TNF-alpha production in whole blood stimulated with Chlamydiae.

Main Results:

  • The C allele frequency was lower in Taiwanese Chinese compared to Western populations.
  • Higher membrane CD14 expression was observed in individuals with the TT genotype compared to CT and CC genotypes.
  • Elevated soluble CD14 levels were found in TT and CT genotypes versus the CC genotype.
  • Significantly higher TNF-alpha production occurred in the TT genotype after Chlamydia stimulation.

Conclusions:

  • The CD14/-260C>T polymorphism is associated with variations in CD14 expression.
  • This genetic variation influences Chlamydia-induced TNF-alpha production, suggesting a role in chlamydia-related inflammatory responses.

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