Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Replicable and recombinogenic RNAs.

Alexander B Chetverin1

  • 1Institute of Protein Research, Russian Academy of Sciences, 142290 Pushchino, Moscow, Russia. alexch@vega.protres.ru

FEBS Letters
|May 29, 2004
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Probing the legitimate initiation of RNA synthesis by Qβ replicase with oligonucleotide primers.

FEBS letters·2024
Same author

Slippage at the initiation of RNA synthesis by Qβ replicase results in a periodic polyG pattern.

FEBS letters·2022
Same author

Alexander Spirin on Molecular Machines and Origin of Life.

Biochemistry. Biokhimiia·2021
Same author

Removal of protein S1 from Escherichia coli ribosomes without the use of affinity chromatography.

Analytical biochemistry·2016
Same author

Structural basis for RNA-genome recognition during bacteriophage Qβ replication.

Nucleic acids research·2015
Same author

Identifying RNA recombination events and non-covalent RNA-RNA interactions with the molecular colony technique.

Methods in molecular biology (Clifton, N.J.)·2014
Same journal

Extending the classical sequence-structure-function paradigm through protein dynamics and context-dependent behavior.

FEBS letters·2026
Same journal

α-Synuclein aggregation landscape from phase separation to neurotoxic intermediates.

FEBS letters·2026
Same journal

Modelling stem cell differentiation related processes-A practical overview for biologists.

FEBS letters·2026
Same journal

Overlapping gut microbiome signatures in aging and disease are characterized by enrichment of medication-associated oral microbes in the gut.

FEBS letters·2026
Same journal

Csk binding to integrin β3 is regulated by tyrosine and threonine phosphorylation of β3.

FEBS letters·2026
Same journal

Mixed-class J-domain protein scaffolds promote expanded aggregate handling and multivalent Hsp70 engagement during functional disaggregase assembly.

FEBS letters·2026
See all related articles

Decades of research on bacteriophage Q RNA replication revealed spontaneous RNA synthesis and sequence rearrangement. These findings impact RNA World hypotheses and in vitro diagnostic tool development.

Area of Science:

  • Molecular Biology
  • Virology
  • Biochemistry

Background:

  • Bacteriophage Q provides a model for studying RNA replication and recombination.
  • Cell-free systems allow detailed investigation of molecular mechanisms.

Purpose of the Study:

  • To summarize 40 years of research on RNA replication and recombination using bacteriophage Q.
  • To highlight the significance of the molecular colony technique.
  • To discuss the implications for the RNA World hypothesis and biotechnological tools.

Main Methods:

  • Cell-free amplification system of bacteriophage Q.
  • Molecular colony technique for analyzing RNA synthesis and rearrangements.

Main Results:

Related Experiment Videos

  • Discovery of the nature of "spontaneous" RNA synthesis by Q replicase.
  • Demonstration of spontaneous RNA sequence rearrangement under physiological conditions.
  • Characterization of RNA replication and recombination pathways.

    • Conclusions:

    • The study provides foundational insights into RNA dynamics.
    • Findings support and refine the RNA World hypothesis.
    • The research facilitates the development of novel in vitro cloning and diagnostic tools.