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Updated: Aug 24, 2026
In-Vivo Calcium Imaging of Sensory Neurons in the Rat Trigeminal Ganglion
Published on: February 9, 2024
Inhibition by bis(7)-tacrine of 5-HT-activated current in rat TG neurons
Jia-Lie Luo1, Jian Zhang, Bing-Cai Guan
1Department of Molecular and Cellular Neurobiology, Tongji Medical College of Huazhong University of Science and Technology, Wuhan 430030, PR China.
Abstract:
Whole-cell recordings were performed on rat trigeminal ganglion (TG) neurons as a modeling experiment to investigate the effect of bis (7)-tacrine, a potential anti-Alzheimer's disease (AD) drug, on 5-HT-induced current (I5-HT). Extracellular 5-HT activated a concentration-dependent inward current that was blocked by ICS 205930. Co-application of bis(7)-tacrine inhibited I5-HT markedly with IC50 at 2 x 10 M. Bis(7)-tacrine shifted the concentration-response curve for I5-HT rightwards with its maximum response unchanged and EC50 increased, suggesting that this inhibition was competitive in nature. Intracellular dialysis of GDP-beta-S did not block bis(7)-tacrine inhibition of I5-HT, which excluded the involvement of G-protein mediation. These results may offer possible modality to understanding the anti-AD mechanism of bis(7)-tacrine.
