Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Simian virus-40 as a gene therapy vector.

Maria Vera1, Puri Fortes

  • 1School of Medicine, Foundation for Applied Medical Research, Division of Gene Therapy, Laboratory of Vectors Development, University of Navarra, Pamplona, Spain.

DNA and Cell Biology
|June 1, 2004
PubMed
Summary

Recombinant Simian virus-40 (SV40) vectors show promise for gene therapy due to efficient cell transduction and persistent expression. Ongoing research addresses challenges like limited cloning capacity for broader therapeutic applications.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Validation of the Nursing Stress Scale: Current Stressors in the Health Care Environment in the USA and Spain.

Western journal of nursing research·2026
Same author

The lncRNA ELDR suppresses tumorigenicity of AML by interfering with DNA replication and chromatin accessibility.

Blood advances·2025
Same author

Selective Translation Under Heat Shock: Integrating HSP70 mRNA Regulation with Cellular Stress Responses in Yeast and Mammals.

Molecular biology of the cell·2025
Same author

Author Correction: Uncovering functional lncRNAs by scRNA-seq with ELATUS.

Nature communications·2025
Same author

Localized molecular chaperone synthesis maintains neuronal dendrite proteostasis.

Nature communications·2024
Same author

Uncovering functional lncRNAs by scRNA-seq with ELATUS.

Nature communications·2024

Area of Science:

  • Virology
  • Gene Therapy
  • Molecular Biology

Background:

  • Simian virus-40 (SV40) is an icosahedral papovavirus.
  • Recombinant SV40 vectors (rSV40) are engineered for gene delivery.
  • SV40 vectors offer advantages like high titer production and transduction of resting/dividing cells.

Purpose of the Study:

  • To review the development and application of SV40 viruses as recombinant vectors for gene transfer.
  • To summarize the structure, genomics, and life cycle of wild-type SV40.
  • To discuss strategies for rSV40 vector production and therapeutic applications.

Main Methods:

  • Review of existing literature on SV40 recombinant vector technology.
  • Analysis of SV40 virus structure, genome, and life cycle.

Related Experiment Videos

  • Discussion of methods for rSV40 vector development, production, and titration.

    • Main Results:

    • rSV40 vectors are nonreplicative, easy to produce at high titers (10^12 IU/ml).
    • They efficiently transduce various cell types, including resting and dividing cells, leading to persistent transgene expression.
    • rSV40 vectors are nonimmunogenic, but possess small cloning capacity and risks of random integration.

    Conclusions:

    • rSV40 vectors are promising gene transfer vehicles with demonstrated potential in preclinical models.
    • Preliminary therapeutic results for genetic and acquired diseases are encouraging.
    • Further modifications are being developed to overcome current limitations for enhanced gene therapy applications.