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[Effect of glia cell line-derived neurotrophic factor on cultured spinal motor neurons from embryonic rat]
Xue-qin Song1, Shu-yu Wu, Chun-yan Li
1Department of Neurology,Second Hospital of Hebei Medical University, Shijiazhuang 050000, China.
Aim:
To observe the effects of various concentrations of gli-a cell line-derived neurotrophic factor ( GDNF) on the growth of cultured spinal motor neurons from embryonic rat.
Methods:
Neurons were isolated from embryonic rat spi-nal ventral tissue and then were primarily cultured in vitro. Morphological feature of neurons was examined by immuno-cytochemical staining with mAb SMI-32. The effect of GDNFon growth of spinal motor neurons was detected by MTT colorimetry.
Results:
GDNF could significantly enhance the survival and neurite outgrowth of rat spinal motor neurons cultured in vitro in dose-dependent tendency.
Conclusion:
Varying concentrations of GDNF can enhance the growth of embryonic rat spinal motor neurons to various extent.
