Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A "virtual gland" method for quantifying epithelial fluid secretion.

Toshiya Irokawa1, Mauri E Krouse, Nam Soo Joo

  • 1Cystic Fibrosis Research Laboratory, Stanford University, Stanford, California 94305-2130, USA.

American Journal of Physiology. Lung Cellular and Molecular Physiology
|June 1, 2004
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Therapeutic potential of synergistic mucociliary clearance for cystic fibrosis airways by β-adrenergic plus cholinergic agonists.

The Journal of clinical investigation·2026
Same author

Development and Validation of Prediction Models for Severe Obstructive Sleep Apnea Based on Periodic Health Examinations.

The clinical respiratory journal·2026
Same author

Epithelial ion transport in spring-lengthened jejunum in a porcine model.

Journal of pediatric surgery·2025
Same author

Durable reconstitution of sinonasal epithelium by transplant of CFTR gene corrected airway stem cells.

bioRxiv : the preprint server for biology·2025
Same author

Calibrating sweat chloride levels to CFTR activity via ETI effects on CF subjects with one or two F508DEL mutations.

Journal of cystic fibrosis : official journal of the European Cystic Fibrosis Society·2024
Same author

The lysogenic filamentous <i>Pseudomonas</i> bacteriophage phage Pf slows mucociliary transport.

PNAS nexus·2024
Same journal

Inhibition of thrombospondin-1 signaling prevents experimental bronchopulmonary dysplasia.

American journal of physiology. Lung cellular and molecular physiology·2026
Same journal

Early Dysanapsis in Experimental Bronchopulmonary Dysplasia: Implications for Lifelong Lung Disease.

American journal of physiology. Lung cellular and molecular physiology·2026
Same journal

Alternative splicing of Toll-like receptor pathway mRNAs in lung immune cells from patients with ARDS.

American journal of physiology. Lung cellular and molecular physiology·2026
Same journal

Hyperoxia and iron homeostasis in developing human airway smooth muscle.

American journal of physiology. Lung cellular and molecular physiology·2026
Same journal

The role of estrogen receptors and house dust mite-induced DNA methylation in a mouse model.

American journal of physiology. Lung cellular and molecular physiology·2026
Same journal

Macrophage in the crosshairs: chemical inhalation and lung immune defense.

American journal of physiology. Lung cellular and molecular physiology·2026
See all related articles

Researchers developed a novel virtual gland (VG) apparatus to measure fluid secretion (Jv) and transepithelial potential (TEP) in cultured epithelial cells. This validated tool provides direct measurements of Jv in Calu-3 cells.

Area of Science:

  • Physiology
  • Biophysics
  • Cell Biology

Background:

  • Accurate measurement of epithelial fluid secretion (Jv) and transepithelial potential (TEP) is crucial for understanding cellular function.
  • Existing methods may have limitations in directly assessing these parameters in cultured epithelial models.
  • Calu-3 cells are a relevant model for studying airway epithelial transport.

Purpose of the Study:

  • To introduce and validate a new apparatus, the virtual gland (VG), for measuring Jv, its composition, and TEP in cultured epithelial cells.
  • To assess the functionality of the VG apparatus using Calu-3 cells under various stimulation and inhibition conditions.
  • To provide direct measurements of Jv and TEP in Calu-3 cells.

Main Methods:

  • Development of the virtual gland (VG) apparatus, creating a 10-microl chamber for cultured epithelial cells on a filter insert.

Related Experiment Videos

  • Optical monitoring of secreted fluid bubbles in an oil reservoir to determine Jv and collection for composition analysis.
  • Measurement of TEP under open circuit conditions in Calu-3 cells cultured within the VG system.

    • Main Results:

    • The VG apparatus successfully measured basal Jv (2.7 ± 0.1 μl/cm²/h) and TEP (-9.2 ± 0.6 mV) in Calu-3 cells.
    • Various agents (forskolin, 1-ethyl-2-benzimidazolinone, thapsigargin) significantly stimulated Jv and TEP, demonstrating the apparatus's sensitivity.
    • Inhibitors (ouabain, bumetanide, acetazolamide, glibenclamide) modulated Jv and TEP, validating the physiological relevance of the measurements.
    • Secreted fluid HCO3- concentration was measured, increasing significantly upon stimulation with forskolin or VIP.

    Conclusions:

    • The virtual gland (VG) apparatus is a validated tool for direct and accurate measurement of fluid secretion rate (Jv) and transepithelial potential (TEP) in cultured epithelial cells.
    • The VG system provides valuable insights into the regulation of epithelial transport mechanisms, as demonstrated in Calu-3 cells.
    • This study establishes a new standard for assessing epithelial fluid transport and ion channel function in vitro.