Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

[New method for quantification of cell swelling using infrared differential interference microscopy].

Ryuichi Nakajima1, Takeshi Nakamura, Hiroyoshi Miyakawa

  • 1Laboratory of Cellular Neurobiology, School of Life Science, Tokyo University of Pharmacy and Life Science. ryuichi@nakajima.com

Nihon Yakurigaku Zasshi. Folia Pharmacologica Japonica
|June 1, 2004
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Ultrasound assessment of true fetal head descent for optimizing operative vaginal delivery.

American journal of obstetrics and gynecology·2026
Same author

The Clinical Details of <i>MYH9</i>-Related Disease and DFNA17 in a Large Japanese Hearing Loss Cohort.

Genes·2026
Same author

Functional and Epigenomic Consequences of <i>DNMT1</i> Variants in Inherited Neurological Disorders.

International journal of molecular sciences·2026
Same author

[Impact of Seasonal Climate Variations on Skin Toxicity Induced by Anti-EGFR Antibody Therapy in Patients with Metastatic Colorectal Cancer].

Gan to kagaku ryoho. Cancer & chemotherapy·2026
Same author

Anesthetic management of Platypnea-orthodeoxia syndrome in a young patient with residual atrial septal defect following congenital heart surgery.

JA clinical reports·2025
Same author

Prevalence of amyloid deposits in synovium and flexor retinaculum, risk factors and asymptomatic cardiac involvement in patients with carpal tunnel syndrome.

The Journal of hand surgery, European volume·2025

We developed a new device using infrared differential interference contrast microscopy (IR-DIC) to quantify brain cell swelling and monitor hippocampal cells in real-time, aiding in edema research.

Area of Science:

  • Neuroscience
  • Cell Biology
  • Biomedical Engineering

Context:

  • Acute brain slices are crucial for studying neurological conditions.
  • Monitoring cellular changes in real-time is essential for understanding brain injury.
  • Existing methods for quantifying brain edema have limitations.

Purpose:

  • To develop a novel device for real-time quantification of cell swelling in acute brain slices.
  • To monitor morphological dynamics of hippocampal cells using infrared differential interference contrast microscopy (IR-DIC).
  • To establish the coefficient of variation (CV) of light intensity as a reliable index for edema formation.

Summary:

  • A new device combining IR-DIC microscopy and computerized image analysis quantifies cell swelling in brain slices.

Related Experiment Videos

  • The coefficient of variation (CV) of light intensity in IR-DIC images effectively measures morphological changes.
  • This method revealed close relationships between edema, light transmittance, CV changes, and field excitatory postsynaptic potentials (fEPSP) during ischemic insult.
  • The device successfully evaluated the neuroprotective effects of mannitol, showing dose-dependent improvement in hippocampal slices after ischemia.

    • Impact:

    • Provides a reliable method for quantifying brain tissue edema.
    • Offers valuable insights into the dynamics of brain swelling during ischemic events.
    • Demonstrates the potential for evaluating neuroprotective agents like mannitol.
    • Enhances the understanding of cellular responses to ischemia and potential therapeutic interventions.