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Development and implementation of a database system to manage a large-scale mouse ENU-mutagenesis program.
Hiroshi Masuya1, Yuji Nakai, Hiromi Motegi
1Mouse Functional Genomics Research Group, RIKEN GSC, 214 Maeda-cho, Totsuka-ku, 244-0804, Yokohama City, Kanagawa, Japan.
Summary
A large-scale mouse mutagenesis program uses comprehensive phenotypic screening and a custom database system (MUSDB) to efficiently manage and analyze genetic data. This approach aids in understanding gene function and inheritance patterns in mice.
Area of Science:
- Genetics
- Genomics
- Animal Models
Background:
- Initiation of a large-scale, genome-wide ENU-mutagenesis program at RIKEN GSC.
- Need for efficient management of extensive phenotypic data from mutant mice.
Purpose of the Study:
- To conduct a comprehensive phenotypic screen of ENU-induced mutant mice.
- To develop and implement a database system for managing the mutagenesis program.
Main Methods:
- Weekly screening of approximately 100 mice using behavioral tests (modified SHIRPA), blood analyses (clinical biochemistry, hemogram), and locomotor activity.
- Development and implementation of a client/server database system (MUSDB) for managing all aspects of the program, including husbandry, procedures, and data.
- Utilizing a large-scale system with 150 client computers.
Main Results:
- MUSDB effectively manages mouse husbandry, mating, ENU injection, phenotypic screening, inheritance tests, and material preservation.
- The system reduced typographical errors and improved operational efficiency through its user-friendly interface.
- Enhanced communication within and between workgroups and facilitated the accumulation of phenotypic and inheritance data.
Conclusions:
- The implemented MUSDB system is crucial for the successful execution of large-scale mouse mutagenesis programs.
- Efficient data management and communication are vital for accumulating comprehensive phenotypic and inheritance data.
- This integrated approach supports genetic research and the discovery of gene function through phenotypic analysis.