Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Microscopy-based multicolor tissue cytometry at the single-cell level.

Rupert C Ecker1, Georg E Steiner

  • 1Competence Center BioMolecular Therapeutics, Vienna, Austria. rupert.ecker@univie.ac.at

Cytometry. Part a : the Journal of the International Society for Analytical Cytology
|June 2, 2004
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Mapping Immunity With Cutting-Edge Spatial Biology and Tissue Cytometry Innovations.

MedComm·2026
Same author

A fusion-based multiomics classification approach for enhanced gene discovery in non-small cell lung cancer.

Bioinformatics advances·2026
Same author

The FKBPL-based therapeutic peptide, AD-01, protects the endothelium from hypoxia-induced damage by stabilising hypoxia inducible factor-α and inflammation.

Journal of translational medicine·2025
Same author

Editorial: Current advances in precision microscopy.

Frontiers in medicine·2025
Same author

A PSA SNP associates with cellular function and clinical outcome in men with prostate cancer.

Nature communications·2024
Same author

Genomic and Phenotypic Biomarkers for Precision Medicine Guidance in Advanced Prostate Cancer.

Current treatment options in oncology·2023
Same journal

The 1st Mediterranean Meeting on Flow Cytometry: Forging New Collaborations Across the Mediterranean and Beyond.

Cytometry. Part A : the journal of the International Society for Analytical Cytology·2026
Same journal

Publication Guidelines for Optimized Multiparameter Immunolabeling Panels (OMIPs).

Cytometry. Part A : the journal of the International Society for Analytical Cytology·2026
Same journal

A Modular High-Parameter Flow Cytometry Framework: Pre-Analytical Optimization and Validation for Clinical Research.

Cytometry. Part A : the journal of the International Society for Analytical Cytology·2026
Same journal

Quantitative Detection of Entotic Cell-In-Cell Structures Using Deformable Segmentation and Deep Learning.

Cytometry. Part A : the journal of the International Society for Analytical Cytology·2026
Same journal

Comparison of Tissue Preparations to Identify and Phenotype T Cells in Human Colorectal Tumor Tissue.

Cytometry. Part A : the journal of the International Society for Analytical Cytology·2026
Same journal

Refractive Index-Correlated Pseudocoloring for Adaptive Color Fusion in Holotomographic Cytology.

Cytometry. Part A : the journal of the International Society for Analytical Cytology·2026
See all related articles

Cytomics offers a new view of life, requiring advanced microscopy. Microscopy-based multicolor tissue cytometry (MMTC) enables quantitative, single-cell analysis of cellular systems for deeper biological insights.

Area of Science:

  • Cellular biology
  • Cytomics
  • Microscopy

Background:

  • Cytomics analyzes cellular systems and their interdependencies.
  • Conventional microscopy lacks quantitative, single-cell, large-population analysis capabilities crucial for cytomics.
  • Advanced microscopy is essential for cytomics research.

Purpose of the Study:

  • To introduce microscopy-based multicolor tissue cytometry (MMTC) as a key technology for cytomics.
  • To outline the techniques and technologies enabling MMTC.
  • To discuss MMTC applications in biological research.

Main Methods:

  • Development of advanced microscopy techniques.
  • Integration of fluorophore technology and sensitive fluorescence detection.
  • Implementation of sophisticated image analysis procedures.

Related Experiment Videos

  • Application of multicolor tissue cytometry (MMTC) for single-cell analysis.

    • Main Results:

    • MMTC addresses limitations of conventional microscopy for cytomics.
    • MMTC facilitates quantitative, observer-independent analysis of large cell populations.
    • Demonstrated applications include leukocyte phenotyping, proliferation marker quantification, and apoptosis assessment in situ.

    Conclusions:

    • MMTC is a vital emerging technology for the cytomics era.
    • Single-cell analysis of tissues using MMTC provides deeper insights into cellular systems.
    • MMTC enables precise quantification of cellular processes within their tissue context.