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Related Experiment Videos

Deformability-based flow cytometry.

Bryan Lincoln1, Harold M Erickson, Stefan Schinkinger

  • 1Institute for Soft Matter Physics, University of Leipzig, Leipzig, Germany.

Cytometry. Part a : the Journal of the International Society for Analytical Cytology
|June 2, 2004
PubMed
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Cell deformability, measured by an optical stretcher, can serve as a novel cell marker. This technique distinguishes normal from cancerous cells and even metastatic from nonmetastatic cancer cells.

Area of Science:

  • Biophysics
  • Cell Biology
  • Biomedical Engineering

Background:

  • Cell elasticity is governed by the cytoskeleton.
  • Alterations in cytoskeletal proteins and networks reflect cellular function, notably in diseases like cancer.
  • Cell deformability presents a potential inherent cell marker linked to cellular function.

Purpose of the Study:

  • To investigate cellular deformability as a novel cell marker.
  • To evaluate the potential of an optical stretcher for high-throughput cell analysis.
  • To differentiate between normal and cancerous cell types based on mechanical properties.

Main Methods:

  • Utilized an optical stretcher, a laser-based instrument, to measure cellular deformability.
  • Incorporated microfluidic cell delivery for potential high-throughput analysis.

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  • Applied optically induced stress to assess cell responses.

    • Main Results:

    • Demonstrated significant differences in deformability between red blood cells and polymorphonuclear cells.
    • Observed cancer cells (MCF-7) stretching approximately five times more than normal cells (MCF-10).
    • Successfully distinguished metastatic from nonmetastatic cancer cells, highlighting optical deformability as a regulated cell marker.

    Conclusions:

    • Preliminary findings suggest a deformability-based cell sorter, analogous to fluorescence-based flow cytometry but without labeling.
    • This approach could potentially aid in diagnosing diseases affecting the cytoskeleton.
    • The technique offers a new avenue for investigating cellular processes impacting cytoskeletal mechanics.