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Related Experiment Videos

Microsphere-induced embolic stroke: an MRI study.

Orna Mayzel-Oreg1, Tsuyoshi Omae, Mark Kazemi

  • 1School of Chemistry, Tel-Aviv University, Tel-Aviv, Israel.

Magnetic Resonance in Medicine
|June 2, 2004
PubMed
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This study characterizes a microsphere-induced stroke model in rats, revealing slower lesion development than the MCAO model. This model offers a valuable tool for stroke research and therapeutic development.

Area of Science:

  • Neuroscience
  • Medical Imaging
  • Experimental Pathology

Background:

  • Efficient stroke therapies are lacking despite extensive research on the middle cerebral artery occlusion (MCAO) model.
  • There is a need for further development and characterization of experimental stroke models to advance stroke research.
  • The multifocal microsphere-induced stroke model in rats is relatively unexplored.

Purpose of the Study:

  • To characterize the multifocal microsphere-induced stroke model in rats using multiparametric MRI.
  • To evaluate infarct development and perfusion deficits over time following microsphere injection.
  • To compare the lesion development kinetics with the established MCAO model.

Main Methods:

  • Induction of microembolic infarction in Sprague-Dawley rats via intracranial injection of polyethylene microspheres.

Related Experiment Videos

  • Multiparametric MRI (diffusion-weighted, perfusion, T(2)-weighted) to assess infarct development at multiple time points (0-48 hours).
  • Histological analysis using 2,3,4-triphenyltetrazolium chloride (TTC) staining to measure infarct volumes.

    • Main Results:

    • Reduced apparent diffusion coefficient (ADC) volumes appeared 0.5-1.0 hr post-injection, reaching 5% of hemisphere volume by 3 hrs.
    • Lesion volume significantly increased to 16% by 12 hrs and remained significant at 24 hrs (15%).
    • Immediate perfusion deficits were observed, with cerebral blood flow index (CBF(i)) decreasing significantly over time, reaching 57% of contralateral values at 24 hrs.

    Conclusions:

    • The microsphere-induced stroke model exhibits slower lesion development compared to the MCAO model.
    • Lesion progression continues up to the 24-48 hour time point in this model.
    • This characterized model provides a valuable platform for investigating stroke pathophysiology and testing therapeutic interventions.