Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

The orofacial formalin test.

Patrick Raboisson1, Radhouane Dallel

  • 1INSERM E 0216, Neurolbiologie de La Douleur Trigéminale, Faculté de Chirurgie Dentaire, Clermont-Ferrand, France.

Neuroscience and Biobehavioral Reviews
|June 3, 2004
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Predicting Pain After Tooth Extraction With Easy-to-Collect Preoperative Variables.

Oral diseases·2026
Same author

Association of parthenolide and salicin as a preventive treatment on a migraine model induced by dural inflammatory soup application.

Pain·2026
Same author

Distinct inflammatory profiles across migraine states: a systematic review and meta-analysis.

The journal of headache and pain·2025
Same author

Astrocytic ion channel Kir4.1 deficit underlies chronic pain.

Neural regeneration research·2025
Same author

Prevalence of Burning Mouth Syndrome in Parkinson's Disease: A Prospective Case-Control Study.

Oral diseases·2025
Same author

Sex influences the progression of cephalic mechanical and light hypersensitivities in a mouse model of chronic migraine.

Headache·2025

The orofacial formalin test reliably quantifies pain and inflammation in rats. This model shows biphasic responses to formalin, useful for analgesic drug screening.

Area of Science:

  • Neuroscience
  • Pharmacology
  • Pain Research

Background:

  • Formalin injection in rats induces a biphasic pain response.
  • The orofacial formalin test models nociception in the trigeminal region.

Purpose of the Study:

  • To establish the orofacial formalin test as a reliable method for quantifying nociception.
  • To assess the validity of face rubbing as a behavioral endpoint.

Main Methods:

  • Subcutaneous injection of formalin into the rat upper lip.
  • Observation and quantification of behavioral responses (face rubbing).
  • Histological examination of tissue inflammation and injury.

Main Results:

  • Formalin injection produced a biphasic, dose-dependent behavioral response (face rubbing).

Related Experiment Videos

  • Response correlated with histological signs of inflammation and injury.
  • The test was sensitive to opioid and non-opioid analgesics.

    • Conclusions:

    • The orofacial formalin test is a valid and reliable model for studying trigeminal nociception.
    • Face rubbing is a relevant endpoint for measuring formalin-induced pain.
    • This model is suitable for evaluating analgesic efficacy.