Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Recent developments in quantitative affinity chromatography.

D J Winzor1

  • 1Department of Biochemistry, University of Queensland, Brisbane, Australia.

Journal of Chromatography
|April 24, 1992
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Theoretical aspects of the quantitative characterization of ligand binding.

Current protocols in protein science·2008
Same author

On the need to consider kinetic as well as thermodynamic consequences of the parking problem in quantitative studies of nonspecific binding between proteins and linear polymer chains.

Biophysical chemistry·2006
Same author

The development of chromatography for the characterization of protein interactions: a personal perspective.

Biochemical Society transactions·2003
Same author

Analysis of thermodynamic non-ideality in terms of protein solvation.

Biophysical chemistry·2002
Same author

A potential role for the analytical ultracentrifuge in the experimental measurement of protein valence.

Analytical biochemistry·2001
Same author

Quantitative affinity chromatography.

Journal of biochemical and biophysical methods·2001
Same journal

Rapid synthesis of isoprenoid diphosphates and their isolation in one step using either thin layer or flash chromatography.

Journal of chromatography·2013
Same journal

[Electrophoresis of radionuclides on cellogel].

Journal of chromatography·2012
Same journal

Frontal affinity chromatography: theory for its application to studies on specific interactions of biomolecules.

Journal of chromatography·2010
Same journal

Cumulative author, subject, compound and special issue indexes. Volumes 601-650 (1992-1993).

Journal of chromatography·1994
Same journal

Determination of the calcium antagonist SIM6080 in plasma by high-resolution gas chromatography.

Journal of chromatography·1994
Same journal

Processing of envelope polypeptides of herpes simplex virus type 1. Demonstration of variation in different cell lines by high-performance liquid chromatography and radioimmunoprecipitation.

Journal of chromatography·1994
See all related articles

Quantitative affinity chromatography advances now enable accurate equilibrium constant evaluation for complex solute-ligand and solute-matrix interactions. This review covers recent developments in high-affinity binding characterization and cellular biospecific adsorption analysis.

Area of Science:

  • Biochemistry
  • Analytical Chemistry
  • Separation Science

Background:

  • Quantitative affinity chromatography (QAC) is a powerful technique for studying molecular interactions.
  • Past research has focused on basic applications, with less emphasis on complex scenarios.

Purpose of the Study:

  • To review recent advancements in QAC over the last decade.
  • To highlight QAC's utility in evaluating equilibrium constants for various interactions.
  • To address common misconceptions regarding affinity matrix suitability.

Main Methods:

  • Survey of literature on QAC developments from the past 10 years.
  • Analysis of QAC adaptations for multivalency, high-affinity interactions, and complex matrices.
  • Review of QAC theory applied to biospecific adsorption in cellular systems.

Related Experiment Videos

Main Results:

  • QAC can effectively evaluate equilibrium constants for solute-ligand and solute-matrix interactions.
  • Highly substituted affinity matrices are suitable for zonal QAC.
  • QAC can characterize high-affinity interactions and biospecific adsorption in cellular systems.

Conclusions:

  • Recent developments have expanded the scope and accuracy of QAC.
  • QAC is a versatile tool for characterizing molecular interactions in various biological contexts.
  • Further application of QAC theory can enhance understanding of cellular adsorption phenomena.