Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Techniques in protein methylation.

Jaeho Lee1, Donghang Cheng, Mark T Bedford

  • 1Deaprtment of Carcinogenesis, Science Park-Research Division, The University of Texas M.D. Anderson Cancer Center, Smithville, TX, USA.

Methods in Molecular Biology (Clifton, N.J.)
|June 3, 2004
PubMed
Summary

Protein methylation, a key posttranslational modification, adds information to peptides. This chapter details methods to assess protein methylation using arginine methyltransferases, including in vivo and in vitro techniques and inhibitors.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Deciphering protein mutation-phenotype linkages from CRISPR-based tiling mutagenesis screens.

Cell systems·2026
Same author

High-coherence narrowband terahertz emission from a radially oscillating plasma oscillator.

Physical review. E·2026
Same author

Radiation-Hardened Perovskite Solar Cells Enabled by Redox-Active V<sub>2</sub>O <i><sub>x</sub></i> Hole Transport Layer for Space Applications.

Small science·2026
Same author

Deep Learning-Based Acoustic Screening for Penetration-Aspiration Events Using Short Voice Recordings.

Dysphagia·2026
Same author

From early prevention to invasive treatment: dental care utilization and outcomes according to gestational age in preterm children.

BMC oral health·2026
Same author

Acquired resistance to the PRMT5 inhibitor confers collateral sensitivity to MEK inhibition in MTAP-null non-small cell lung cancer.

bioRxiv : the preprint server for biology·2026

Area of Science:

  • Biochemistry and Molecular Biology
  • Posttranslational Modifications

Background:

  • Protein methylation is a crucial posttranslational modification affecting peptide information content.
  • It regulates vital cellular processes such as protein trafficking, transcription, and protein-protein interactions.
  • Arginine and lysine residues, as well as carboxy-termini, are common sites for methylation.

Purpose of the Study:

  • To illustrate diverse approaches for assessing protein methylation.
  • To highlight the role of arginine methyltransferases in this process.
  • To demonstrate the application of small molecule inhibitors in studying protein methylation.

Main Methods:

  • Description of in vivo methylation techniques.
  • Detailed explanation of in vitro methylation assays.

Related Experiment Videos

  • Demonstration of using small molecule inhibitors to study protein methylation.

    • Main Results:

    • The chapter provides a comprehensive overview of methods to evaluate protein methylation.
    • It emphasizes the utility of arginine methyltransferases as a model system.
    • Practical examples of assessing methylation in different contexts are presented.

    Conclusions:

    • Various techniques, both in vivo and in vitro, are effective for studying protein methylation.
    • Arginine methyltransferases serve as valuable tools for understanding methylation mechanisms.
    • Small molecule inhibitors offer a means to probe the functional consequences of protein methylation.