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Related Experiment Videos

The decrease in FlaA observed in a flaB mutant of Borrelia burgdorferi occurs posttranscriptionally.

M A Motaleb1, Melanie S Sal, Nyles W Charon

  • 1Department of Microbiology, Immunology, and Cell Biology, West Virginia University, Box 9177, Robert C. Byrd Health Sciences Center, Morgantown, WV 26506-9177, USA. ncharon@hsc.wvu.edu

Journal of Bacteriology
|June 4, 2004
PubMed
Summary

The Lyme disease bacterium Borrelia burgdorferi relies on periplasmic flagella for motility. Lack of the FlaB protein reduces FlaA protein levels, impacting bacterial movement.

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Area of Science:

  • Microbiology
  • Molecular Biology
  • Biochemistry

Background:

  • Borrelia burgdorferi, the Lyme disease bacterium, is a motile spirochete.
  • Periplasmic flagella are crucial for the characteristic wavy shape and motility of B. burgdorferi.
  • These flagella are composed of major filament protein FlaB and minor protein FlaA.

Purpose of the Study:

  • To investigate the structure and regulation of B. burgdorferi periplasmic flagella.
  • To confirm FlaA as a flagellar protein and assess its role in flagellar morphology.
  • To understand motility gene regulation in B. burgdorferi, particularly the effect of flaB mutations.

Main Methods:

  • Immunoprecipitation was used to confirm FlaA as a flagellar protein.
  • Analysis of a flaB insertion mutant examined effects on other motility gene products.

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  • Real-time reverse transcriptase PCR and protein stability assays were employed.

    • Main Results:

    • Immunoprecipitation provided further evidence for FlaA as a flagellar protein.
    • In a flaB mutant, FlaA protein levels decreased by 13% without affecting flaA mRNA levels.
    • Protein stability analysis indicated increased turnover of FlaA in the flaB mutant.

    Conclusions:

    • B. burgdorferi FlaA does not influence the overall helical shape of periplasmic flagella.
    • The absence of FlaB negatively impacts FlaA protein levels, likely through translational control or protein turnover.
    • This study sheds light on the regulation of motility in B. burgdorferi.