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Chemotactic activities of peripheral blood polymorphonuclear leukocytes and peritoneal exudate polymorphonuclear
S Sasagawa1, Y Satow, K Suzuki
1Radiation Effects Research Foundation, Hiroshima, Japan.
Abstract:
Chemotactic responsiveness to fMet-Leu-Phe in concentrations of 10(-8) to 10(-4) M in the Boyden chamber was compared between peritoneal exudate cells (PEC) and polymorphonuclear leukocytes (PMN) isolated from peripheral blood, between MRL/Mp-+/+ (MRL-+/+) and MRL/Mp-lpr/lpr (MRL-lpr/lpr) mice, and between young (6-9 week old) and aged (16-24 week old) mice. Chemotactic responsiveness of PEC did not differ between MRL-+/+ and MRL-lpr/lpr, and young and aged mice. While, PMN showed greater chemotaxis in aged MRL-+/+ mice than that in aged MRL-lpr/lpr mice. These results suggest that chemotactic responsiveness of PMN differ from that of PEC which is assumed to be preactivated by an inflammatory agent injected into the peritoneal cavity to elicit cells. Less responsiveness of PMN to the bacterial origin peptide might relate to the autoimmune disease of this murine model.
Insights
Chemotactic responsiveness of peritoneal exudate cells (PEC) was consistent across mouse strains and ages. However, polymorphonuclear leukocytes (PMN) from aged autoimmune mice showed reduced chemotaxis compared to controls.
Area of Science:
- Immunology
- Cell Biology
- Autoimmune Diseases
Background:
- Chemotaxis is crucial for immune cell function.
- Murine models of autoimmune disease, like MRL-lpr/lpr mice, exhibit immune dysregulation.
- Age can influence immune cell responsiveness.
Purpose of the Study:
- To compare the chemotactic responsiveness of peritoneal exudate cells (PEC) and peripheral blood polymorphonuclear leukocytes (PMN).
- To investigate differences in chemotaxis between MRL-+/+ and MRL-lpr/lpr mice.
- To assess the impact of age on immune cell chemotaxis in these mouse models.
Main Methods:
- Utilized the Boyden chamber assay to measure chemotactic responsiveness.
- Tested fMet-Leu-Phe peptide at concentrations from 10(-8) to 10(-4) M.
- Isolated PEC and PMN from young and aged MRL-+/+ and MRL-lpr/lpr mice.
Main Results:
- PEC chemotactic responsiveness was uniform across all groups (MRL-+/+, MRL-lpr/lpr, young, aged).
- PMN from aged MRL-+/+ mice exhibited significantly greater chemotaxis than PMN from aged MRL-lpr/lpr mice.
- Aged MRL-lpr/lpr mice showed diminished PMN chemotactic responsiveness.
Conclusions:
- PMN chemotaxis differs significantly from PEC responsiveness, potentially due to PEC preactivation.
- Reduced PMN chemotaxis in aged MRL-lpr/lpr mice may be linked to their autoimmune condition.
- These findings highlight specific immune cell deficits in a murine model of autoimmune disease.