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A simple and economical strategy for downstream processing of specific antibodies to human transferrin from egg yolk
V Ntakarutimana1, P Demedts, M van Sande
1Laboratory of Medical Biochemistry, Universitaire Instelling Antwerpen (U.I.A.), Wilrijk, Belgium.
A rapid and economical procedure for extraction of antibodies from egg yolk is described. Laying hens were immunized with human transferrin and extracts of egg-yolk were purified with a procedure based on affinity chromatography. The resulting purified antibodies were evaluated in a nephelometric system for the assay of transferrin in human sera. The results agreed closely with those obtained with a commercially available anti-human transferrin serum from rabbits.
A rapid and economical procedure for extraction of antibodies from egg yolk is described. Laying hens were immunized with human transferrin and extracts of egg-yolk were purified with a procedure based on affinity chromatography. The resulting purified antibodies were evaluated in a nephelometric system for the assay of transferrin in human sera. The results agreed closely with those obtained with a commercially available anti-human transferrin serum from rabbits.