Related Experiment Videos
Effective method for synthetic peptide immobilization that increases the sensitivity and specificity of ELISA
V S Ivanov1, Z K Suvorova, L D Tchikin
1Shemyakin Institute of Bioorganic Chemistry, Academy of Sciences of Russia, Moscow.
Journal of Immunological Methods
|August 30, 1992
Abstract:
A procedure is described for the immobilization of synthetic peptide antigens on a plastic solid phase for performing ELISA. The use of a streptavidin-biotinylated peptide system for coating microplates with peptide antigen markedly increased both the sensitivity and the specificity compared to a standard ELISA based on synthetic peptides. The procedure was used for the detection of HIV-1-specific antibodies.