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miRNA Expression Analyses in Prostate Cancer Clinical Tissues
11:29

miRNA Expression Analyses in Prostate Cancer Clinical Tissues

Published on: September 8, 2015

Prostein expression is highly restricted to normal and malignant prostate tissues

Michael Kalos1, Jon Askaa, Bonnie L Hylander

  • 1Corixa Corporation, Seattle, Washington 98104, USA.

The Prostate
|June 4, 2004
PubMed
Abstract

Insights

Prostein protein is highly specific to prostate tissues. A new antibody, 10E3-G4-D3, confirms prostein

Area of Science:

  • Biochemistry
  • Immunology
  • Oncology

Background:

  • Prostein is a prostate-specific molecule identified at the mRNA level.
  • A murine monoclonal antibody (10E3-G4-D3) was developed to detect prostein protein.
  • The antibody's expression was evaluated in various normal and tumor tissues.

Purpose of the Study:

  • To characterize the anti-prostein monoclonal antibody 10E3-G4-D3.
  • To determine the protein expression pattern of prostein in normal and malignant tissues.
  • To assess the potential of prostein as a diagnostic biomarker and therapeutic target for prostate cancer.

Main Methods:

  • Generation of a murine anti-prostein monoclonal antibody (10E3-G4-D3) using recombinant prostein.
  • Characterization of the antibody using ELISA, FACS, and Western blot analyses.
  • Immunohistochemistry (IHC) was employed to analyze prostein expression in tissue samples.

Main Results:

  • The 10E3-G4-D3 antibody specifically recognizes a linear intracellular epitope of prostein.
  • Prostein protein is expressed in most normal and malignant prostatic tissues, irrespective of grade or metastatic status.
  • No prostein protein expression was detected in approximately 4,700 samples from other normal and malignant tissues.

Conclusions:

  • Prostein exhibits exceptional specificity for prostate tissues.
  • The 10E3-G4-D3 antibody shows potential as a diagnostic biomarker for prostate cancer.
  • Prostein is a promising novel target for developing prostein-specific antibody and T-cell based therapies for prostate cancer.

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