Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Excitation-emission fluorimeter based on linear interference filters.

Michael Gouzman1, Nadia Lifshitz, Serge Luryi

  • 1Department of Electrical and Computer Engineering, State University of New York at Stony Brook, Stony Brook, New York 11794-2350, USA.

Applied Optics
|June 5, 2004
PubMed
Summary

This study introduces a novel excitation-emission (EE) fluorimeter utilizing linear interference filters (LIFs). This compact instrument enables simultaneous spectral characterization of excitation and emission properties for various fluorescent materials.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Synergistic Effect of Poly(ethylenephosphoric Acid) and Cerium in Bone Substitute Composites on Tissue Response and Bone Remodeling.

International journal of molecular sciences·2025
Same author

Matere bond identification in non-crystalline materials by using X-ray photoelectron spectroscopy.

Chemical science·2025
Same author

Revising Model Reactions in Plasmonic Chemistry: From Nitrothiophenol Coupling to Alkoxyamine Homolysis.

ACS catalysis·2025
Same author

Ultrafast synthesis of zirconium-porphyrin framework nanocrystals from alkoxide precursors.

Cell reports. Physical science·2024
Same author

Dispersant and Protective Roles of Amphiphilic Poly(ethylene phosphate) Block Copolymers in Polyester/Bone Mineral Composites.

International journal of molecular sciences·2023
Same author

"Functional upcycling" of polymer waste towards the design of new materials.

Chemical Society reviews·2023

Area of Science:

  • Analytical Chemistry
  • Spectroscopy
  • Optical Instrumentation

Background:

  • Excitation-emission (EE) fluorimeters are crucial for spectral characterization.
  • Traditional EE instruments often use bulky monochromators.
  • Simultaneous excitation and emission spectral analysis requires precise wavelength selection.

Purpose of the Study:

  • To design and evaluate a novel EE fluorimeter.
  • To implement lightweight and compact linear interference filters (LIFs) for wavelength selection.
  • To demonstrate simultaneous spectral characterization capabilities.

Main Methods:

  • Developed an EE fluorimeter employing two linear interference filters (LIFs).
  • Utilized computer-controlled linear step motors for mechanical shifting and spectral tuning of LIFs.

Related Experiment Videos

  • Tested the instrument's performance using fluorescent spectra of various dyes and mixtures.

    • Main Results:

    • The developed EE fluorimeter successfully performs simultaneous spectral characterization.
    • Linear interference filters offer a compact and lightweight alternative to monochromators.
    • The instrument demonstrated effective performance with diverse fluorescent samples.

    Conclusions:

    • The LIF-based EE fluorimeter provides an effective and compact solution for spectral analysis.
    • This design advances EE fluorimetry by enabling simultaneous excitation and emission characterization.
    • The instrument shows potential for various applications in fluorescence spectroscopy.