Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Proteomics for protein expression profiling in neuroscience.

Willard M Freeman1, Scott E Hemby

  • 1Department of Pharmacology, Yerkes National Primate Research Center, Neuroscience Division, Emory University School of Medicine, Atlanta, Georgia 30329, USA.

Neurochemical Research
|June 5, 2004
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Development and validation of a UPLC-MS/MS method for real-time neuropharmacokinetic monitoring of iboga alkaloids in rat brain.

Journal of pharmaceutical and biomedical analysis·2026
Same author

Low-dose tamoxifen ameliorates ovariectomy-induced metabolic and immune dysfunction.

GeroScience·2026
Same author

Single Cell RNAseq Analysis of Thyroid Hormone Effects on Retinal Glial Cells.

Aging and disease·2025
Same author

Hepatocyte MLKL Drives Obesity-Driven Hepatocellular Carcinoma Progression via Mitochondrial Dysfunction Independent of Necroptosis in MASLD.

bioRxiv : the preprint server for biology·2025
Same author

Necroptosis induced by MLKL overexpression in liver triggers cellular senescence and leads to chronic inflammation and fibrosis.

GeroScience·2025
Same author

Neuronal Necroptosis Drives Neuroinflammation and Cognitive Decline Independent of Neuronal Cell Death.

Aging and disease·2025

Proteomics offers neuroscientists powerful new tools, but challenges like small sample sizes and rare proteins require careful method selection. This review explores key proteomic techniques for advancing neuroscience research.

Area of Science:

  • Neuroscience
  • Proteomics
  • Biochemistry

Background:

  • Proteomics technology is transitioning from theoretical to practical application.
  • Neuroscientists face unique challenges, including limited sample sizes, sample heterogeneity, and detecting rare or hydrophobic proteins.

Purpose of the Study:

  • To review protein isolation, fractionation, and separation techniques for neuroproteomics.
  • To discuss methods for quantifying relative protein expression between samples.
  • To evaluate different proteomic approaches based on coverage, membrane protein detection, resource needs, and quantitative reliability.

Main Methods:

  • Two-dimensional electrophoresis (2-DE)
  • Mass spectrometry-based proteomics
  • Differential gel electrophoresis (2-DIGE)

Related Experiment Videos

  • Isotope-coded affinity tag (ICAT) labeling

    • Main Results:

    • The review examines various proteomic methods applicable to neuroscience research.
    • Challenges in neuroproteomics include sample limitations and protein characteristics.
    • Different techniques offer varying capabilities in proteome coverage and quantitative accuracy.

    Conclusions:

    • Despite existing challenges, neuroproteomics holds significant future promise.
    • Careful selection of proteomic methods is crucial for successful neuroscience applications.
    • Advancements in proteomics will drive discoveries in brain function and disease.