Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Methodological considerations regarding single-cell gene expression profiling for brain injury.

Jason E Davis1, James H Eberwine, David A Hinkle

  • 1The Head Injury Center, Department of Neurosurgery, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania, USA.

Neurochemical Research
|June 5, 2004
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Mechanical deformation explains distinct neuroimaging patterns and etiologies in brain trauma.

NeuroImage·2026
Same author

Reversibly-sealable microfluidic platform for multi-molecule gradient delivery to large adherent cell cultures.

Biomedical microdevices·2026
Same author

Microglia modulate concussion biomarkers and cognitive recovery in male mice.

bioRxiv : the preprint server for biology·2026
Same author

Subconcussive Preconditioning Modifies Acute Microglial Response and Improves Cognitive Outcomes Post-Concussion.

Journal of neurotrauma·2026
Same author

Microfluidic nanomagnetically isolated neuron- and astrocyte-derived extracellular vesicles to differentiate Lewy body and Alzheimer's disease.

Npj biosensing·2026
Same author

A Proposed Novel Neurovascular Mechanism for Brain Network Dysfunction After Traumatic Injury.

Annals of biomedical engineering·2026

Genomic microarrays and single-cell mRNA amplification reveal molecular insights into central nervous system (CNS) injury and disease. These techniques enhance gene expression profiling for neuropathology research, particularly in traumatic brain injury models.

Area of Science:

  • Genomics
  • Neuroscience
  • Molecular Biology

Background:

  • Genomic microarrays are increasingly utilized across biological disciplines.
  • Discoveries in central nervous system (CNS) injury and disease have been facilitated by this technology.
  • Cellular heterogeneity in the brain presents challenges for gene expression analysis.

Purpose of the Study:

  • To review the application of genomic microarrays and single-cell mRNA amplification in CNS injury and disease.
  • To highlight advancements in gene expression profiling for understanding neuropathology.
  • To discuss methodological improvements for microarray reproducibility and data analysis.

Main Methods:

  • Utilizing genomic microarrays for gene expression profiling.
  • Employing single-cell mRNA amplification techniques to address cellular heterogeneity.

Related Experiment Videos

  • Applying these methods to experimental models of traumatic brain injury.

    • Main Results:

    • Gene expression profiling provides molecular and cellular insights into CNS injury and disease.
    • Single-cell mRNA amplification overcomes limitations of brain tissue heterogeneity.
    • Advancements are improving microarray reproducibility and data analysis.

    Conclusions:

    • Genomic microarrays coupled with single-cell mRNA amplification offer powerful tools for CNS research.
    • These technologies are crucial for advancing our understanding of neuropathology.
    • Ongoing methodological developments promise more robust and insightful research in traumatic brain injury.