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Updated: Aug 24, 2026

Activated Cross-linked Agarose for the Rapid Development of Affinity Chromatography Resins - Antibody Capture as a Case Study
Published on: August 16, 2019
Modeling of monolith-supported affinity chromatography
Francisco J Montes Sanchez1, Eva Martin del Valle, Miguel A Galan Serrano
1Department of Chemical and Materials Engineering, University of Alabama in Huntsville, Huntsville, Alabama 35899, USA.
Abstract:
Ceramic monoliths have been used successfully as active support for affinity chromatography (1). A mathematical model was developed to simulate the adsorption-elution experimental behavior of asparaginase in an agarose-coated monolith support. The computer-based model allows precise determination of experimental parameters. Because of the simple geometry of ceramic monoliths used as support, the mathematical model can be used to design adsorption/elution cycles for the large throughput separation of biomolecules.
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