Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Human mesenchymal stem cells tissue development in 3D PET matrices.

Warren L Grayson1, Teng Ma, Bruce Bunnell

  • 1Department of Chemical and Biomedical Engineering, FAMU-FSU College of Engineering, Florida State University, Tallahassee, Florida, USA.

Biotechnology Progress
|June 5, 2004
PubMed
Summary

Human mesenchymal stem cells (hMSCs) cultured in 3D scaffolds show distinct tissue development patterns compared to 2D cultures. These findings offer insights for designing effective 3D scaffolds for tissue engineering applications.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Parallel Recordings of Transmembrane hERG Channel Currents Based on Solvent-Free Lipid Bilayer Microarray.

Micromachines·2021
Same author

Characterization and association of bacterial communities and nonvolatile components in spontaneously fermented cow milk at different geographical distances.

Journal of dairy science·2021
Same author

Characterization of the complete chloroplast genome of <i>Cucun mismelo</i> L. var. <i>Agrestis</i> Naud.

Mitochondrial DNA. Part B, Resources·2021
Same author

α-ketoglutarate delays age-related fertility decline in mammals.

Aging cell·2021
Same author

DNA-PKcs: A Multi-Faceted Player in DNA Damage Response.

Frontiers in genetics·2021
Same author

Clinical Efficacy of Vertical or Parallel Technique of a Micro-Locking Plate for Treatment of Dubberley B-Type Capitellar Fractures.

Orthopaedic surgery·2021

Area of Science:

  • Biomaterials Science
  • Cell Biology
  • Tissue Engineering

Background:

  • Human mesenchymal stem cells (hMSCs) are promising for tissue engineering.
  • Three-dimensional (3D) culture environments are crucial for mimicking in vivo conditions.
  • Poly(ethylene terephthalate) (PET) fibrous matrices offer a potential scaffold material.

Purpose of the Study:

  • To investigate 3D hMSC tissue development in PET fibrous scaffolds.
  • To compare hMSC behavior in 3D scaffolds versus 2D cultures.
  • To assess the potential of 3D hMSC constructs for multi-lineage differentiation.

Main Methods:

  • hMSCs were seeded onto 3D PET scaffolds and cultured for over one month.
  • Cell proliferation, extracellular matrix (ECM) deposition, and protein expression were analyzed.

Related Experiment Videos

  • Immunohistochemistry and Western blotting were used to assess integrin and paxillin expression.
  • Multi-lineage differentiation into osteoblasts and adipocytes was performed.

    • Main Results:

    • hMSC proliferation in 3D scaffolds was lower than in 2D cultures but resulted in extensive ECM deposition.
    • hMSCs organized ECM proteins into aligned fibrils influenced by PET fiber orientation.
    • Differential expression of integrins (α2β1, α5β1, αVβ3) and paxillin was observed between 3D and 2D cultures.
    • Successful differentiation of 3D hMSC constructs into osteoblasts and adipocytes was demonstrated.

    Conclusions:

    • 3D PET scaffolds support distinct hMSC tissue development patterns compared to 2D cultures.
    • Scaffold architecture influences hMSC behavior and ECM organization.
    • These findings provide valuable information for the design of 3D scaffolds for tissue engineering.